Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves Phe1-|-Val, Gln4-|-His, Glu13-|-Ala, Ala14-|-Leu, Leu15-|-Tyr, Tyr16-|-Leu, Gly23-|-Phe, Phe24-|-Phe and Phe25-|-Tyr bonds in the B chain of insulin
BiSSCat v1.06.20http://bisscat.org/ Developed by Masaaki Kotera, Department of Biochemistry, Trinity College Dublin. Science Foundation of Ireland is gratefully acknowledged.